《植物生理学报》 2010, 46(4): 370-374

白桦单细胞培养体系的建立

占爱瑶, 由香玲, 詹亚光*, 付丽楠, 何之龙, 王翀

东北林业大学生命科学学院, 哈尔滨150040

通信作者：詹亚光；E-mail: yaguangzhan@126.com；Tel: 0451-82191 752

摘　要：

以白桦花药(单核靠边期)、种子(萌发)及再生苗的茎段诱导愈伤组织, 在 NT1、NT2 和 B5 三种不同的培养基中进行 悬浮培养, 筛选出优良的白桦悬浮体系, 而后进行单细胞分离的结果表明, 白桦花药愈伤组织在B5培养基上生长迅速, 分散性好, 适合于细胞分离。分离得到的单细胞用不同方式培养, 建立了白桦单细胞两步培养法。

关键词：白桦; 悬浮培养; 优良悬浮体系; 单细胞培养

收稿：2009-12-23   修定：2010-03-17

资助：东北林业大学本科生创新项目(091022532)和国家自然科学基金(30872045)。

Establishment of Single Cell Culture of White Birch (Betula platyphylla Suk.)

ZHAN Ai-Yao, YOU Xiang-Ling, ZHAN Ya-Guang*, FU Li-Nan, HE Zhi-Long, WANG Chong

College of Life Sciences, Northeast Forestry University, Harbin 150040, China

Corresponding author: ZHAN Ya-Guang; E-mail: yaguangzhan@126.com; Tel: 0451-82191 752

Abstract:

In this study, calli of Betula platyphylla were induced from three kinds of explants: anther (microspores in monokaryotic stage), the seedling, and the stem of in vitro cultured plant. The best growth of calli induced from anther was obtained in the B5 medium when they were suspended in three media: NT1, NT2 and B5, respectively, also in that medium the anther calli shown good dispersivity in liquid medium, which was suited to single cell separation. The single cell was cultured in different ways, and the white birch two-step single cell culture was established.

Key words: Betula platyphylla; suspension culture; fine suspension system; single cell culture